Tech Note

Fluorescent Western Blotting - A Guide to Multiplexing



Improvements in imaging and fluorescent probes provide scientists with enhanced tools to maximize the utility of western blot technology. Fluorescent western blotting provides accurate, quantitative results, stable signals, and the ability to evaluate multiple protein targets on a single blot, which is known as multiplexing.

With the Invitrogen™ iBright™ FL1000 Imaging System, one can perform up to a 4-plex fluorescent western blot with the appropriate experimental setup. In this Tech Note, we share best practices and tips for success in multiplex fluorescent western blotting.

Download this Tech Note for advice on:

  • Getting started, important considerations to minimize background fluorescence
  • Filter sets and fluorophore selection
  • Protocol setup
  • Troubleshooting problematic scenarios